5/30/2023 0 Comments Introduction to light microscopy![]() ![]() ![]() They are often enhanced by methods to increase the contrast in samples, such as phase contrast, differential interference contrast, and darkfield illumination. For image acquisition, these microscopes are typically fitted with RGB cameras to acquire colour images using brightfield illumination. Standard light microscope – These are the basic systems for every-day use, mainly in the area of medical and histology imaging. Therefore, knowledge of the general technologies and the hardware available is an essential part of your imaging expertise and experimental plan. The most crucial thing is that you fully understand what a given system can deliver and more importantly what it cannot achieve. studying the high resolution 3-D morphology of a particular cell type by confocal microscopy as well as measuring quick cellular events such as 2-D timelapse images on a high-end widefield microscope in the same experimental system. Combination of complementary types of microscopes often helps to answer more complex experimental questions, e.g. ![]() To help you get the best out of your imaging experiment by making the right choice of microscope, this article will highlight the main features of the different, basic light microscopes and give some recommendations (and next week we look at different confocal systems). Manufacturers offer an ever increasing range of light microscopes- some of which may already be available in your research institutes. Before you even start preparing your samples, you should think about the choice of microscope for image acquisition.
0 Comments
Leave a Reply. |